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1996-02-27
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Document 0071
DOCN M9630071
TI Expression and quantification of IgG and IgM molecules on the surface of
lymphocytes of cattle infected with bovine leukaemia virus.
DT 9603
AU Levkut M; Ponti W; Soligo D; Quirici N; Rocchi M; Lambertenghi Deliliers
G; Department of Pathological Anatomy, University of Veterinary;
Medicine, Kosice, Slovakia.
SO Res Vet Sci. 1995 Jul;59(1):45-9. Unique Identifier : AIDSLINE
MED/96131126
AB Immunogold-labelled antibodies were used with scanning electron
microscopy (SEM) and fluorescent-labelled antibodies were used with flow
cytometry (FACS) to evaluate the expression and quantity of IgG and IgM
molecules on the surface of the lymphocytes of cattle infected with
bovine leukaemia virus (BLV). The BLV-infected animals were divided
serologically and haematologically into groups with (BLV+PL+) and
without (BLV+PL-) persistent lymphocytosis (PL). The percentage of
IgM-bearing cells was significantly higher in the BLV+PL+ group than in
the BLV-PL- and BLV+PL- groups by FACS. There was a significantly higher
percentage of IgG-bearing cells in the BLV+PL+ group than in the BLV-PL-
and BLV+PL- groups by SEM, but no differences were found by FACS. A
significantly higher intensity of IgM expression was observed in the
BLV+PL+ group by SEM. A higher intensity of IgG expression in some
animals was detected only by SEM. An increase in the number of larger
IgM cells were observed in the BLV+PL- and BLV+PL+ groups by SEM. The
SEM analysis was more sensitive than FACS in this experiment.
DE Animal Antibodies, Monoclonal Antibodies, Viral/IMMUNOLOGY/ISOLATION &
PURIF Antigens, Surface/*BIOSYNTHESIS Cattle Enzootic Bovine
Leukosis/*IMMUNOLOGY Flow Cytometry/VETERINARY IgG/*BIOSYNTHESIS
IgM/*BIOSYNTHESIS Lymphocytes/*IMMUNOLOGY Microscopy, Electron,
Scanning/VETERINARY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).